===== Origami: Space Invader =====

==== Freezer mix ====

In 0.5mL tubes:

  * I'll make **9x16 = ~~=9*16~~** of those:

^  //Freezer Mix of 16 Staples (300uL at 2500nM each) //  ^^^^^^
^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
^  Freezer Mix of 16 Staples (2500nM) ^^^^  300 ^^
^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ 
^ staple  |  50000 |  16 |  2500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |

  * I'll make 1 of this:

^  //Freezer Mix of 12 Staples (300uL at 2500nM each) //  ^^^^^^
^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
^  Freezer Mix of 12 Staples (5000nM) ^^^^  300 ^^
^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ 
^ staple  |  50000 |  12 |  2500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |


==== Fridge mix ====

In 0.5mL tubes:

  * I'll make 1 of these:

^  //Fridge Mix of 80 Staples (500uL at 500nM) //  ^^^^^^
^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
^  Fridge Mix of 80 Staples (1000nM) ^^^^  500 ^^
^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ 
^ Freezer Mix of 16 Staples (2500nM)  |  2500 |  5 |  500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |

  * I'll make 1 of these:

^  //Fridge Mix of 76 Staples (500uL at 500nM) //  ^^^^^^
^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
^  Fridge Mix of ~~=4*16+12~~ Staples (1000nM) ^^^^  500 ^^
^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ 
^ Freezer Mix of 16 Staples (2500nM)  |  2500 |  4 |  500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
^ Freezer Mix of 12 Staples (2500nM)  |  2500 |  1 |  500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |

==== M13 fridge tube ====

^  //10nM p7249 M13mp18 fridge tube 500mL//  ^^^^^^
^  Product  ^ Concentration (mM)  ^  Number  ^  Target concentration (mM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color> ^
^  10nM p7249 M13mp18 ^^^^  500 ^^
^  H<sub>2</sub>O ^^^^  **~~=r2c1 - sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ 
^ 100nM Tilibit p7249 M13mp48  |  100 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |

Store the rest of the Tilibit p7249 M13mp18 2ml 100nM in Freezer (-20°C)
==== Origami mix ====
In a 0.5mL tube:

^  //Origami Mix (500uL) //  ^^^^^^
^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
^  Origami Mix ^^^^  500 ^^
^  H<sub>2</sub>O ^^^^  ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ 
^ TAE Mg 10x  |  10 |  1 |  1 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
^ Fridge M13  |  10 |  1 |  1 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
^ Fridge Mix of 80 Staples  |  500 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
^ Fridge Mix of 76 Staples  |  500 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |