**PM2 2021.03.04 WaterWings cooking test** 


**goal** : Cook the water wings and observe the docking blob on the AFM.

**Cooking** 

Master Mix 

//docking staples design ://
 


Vtot = 60 uL
| product | concentration | target concentration | volume |
| H20 | | | 10uL|
| TAE Mg 10X | 10 nM | 1 nM | 10 uL |
| Fridge M13 | 10 nM | 1 nM | 10 uL |
| Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 10uL |
| for each 30 docking staples | 500 nM | 10nM | 2 uL |



tube WW A : 30uL Master Mix
tube WW B : 30uL Master Mix


5 min 65deg
65 -> 55 : 15 min per degree 
55 -> 40 : 140 min per degree
hold at 25 deg

TODO CHECK THE TEMPERATURE CURVE



** Afm **


**sample 1 - Na 2 times, TAE 2 times** 
 WW A 25uL
Na 20uL 3 min
Na 20uL 3 min
TAE 1X 20uL 3 min
TAE 1X 20uL 3 min
TAE 1X 60uL

images on the AFM PC

**sample 2 - Na, TAE** 
 WW B 25uL
Na 20uL 3 min
TAE 1X 20uL 3 min
TAE 1X 60uL

forgot to save the image ? 



**sample 3 - direct** 
 WW A 25uL
Na 20uL 3 min

images on the AFM PC



**Conclusion**

Sample 3 has a lot more of origamis compared to sample 1  ( about several times more) 
-> rising loose many origamis

Some stacking between the water wings : two origamis stuck together