**021.03.05_pm3_water_wings_melt**


**Goal : do a melt curve of the WW origami**

Master Mix : 
Vtot =200 uL
| product | concentration | target concentration | volume |
| TAE Mg 10X | 10 nM | 1 nM | 20 uL |
| Fridge M13 | 10 nM | 1 nM | 20 uL |
| Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 20uL |
| for each 30 docking staples | 500 nM | 10nM | 4 uL |
| Sybr 20X | | | 10 uL|
| H20 | | | 10uL|


8 pcr tube of 20uL master mix

melt curve 
total time 11h