Table of Contents

Origami: Space Invader

Freezer mix

In 0.5mL tubes:

Freezer Mix of 16 Staples (300uL at 2500nM each)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Freezer Mix of 16 Staples (2500nM) 300
H2O
staple 50000 16 2500
Freezer Mix of 12 Staples (300uL at 2500nM each)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Freezer Mix of 12 Staples (5000nM) 300
H2O
staple 50000 12 2500

Fridge mix

In 0.5mL tubes:

Fridge Mix of 80 Staples (500uL at 500nM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Fridge Mix of 80 Staples (1000nM) 500
H2O
Freezer Mix of 16 Staples (2500nM) 2500 5 500
Fridge Mix of 76 Staples (500uL at 500nM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Fridge Mix of Staples (1000nM) 500
H2O
Freezer Mix of 16 Staples (2500nM) 2500 4 500
Freezer Mix of 12 Staples (2500nM) 2500 1 500

M13 fridge tube

10nM p7249 M13mp18 fridge tube 500mL
Product Concentration (mM) Number Target concentration (mM) Volume (uL) Vol*nb
10nM p7249 M13mp18 500
H2O
100nM Tilibit p7249 M13mp48 100 1 10

Store the rest of the Tilibit p7249 M13mp18 2ml 100nM in Freezer (-20°C)

Origami mix

In a 0.5mL tube:

Origami Mix (500uL)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Origami Mix 500
H2O
TAE Mg 10x 10 1 1
Fridge M13 10 1 1
Fridge Mix of 80 Staples 500 1 10
Fridge Mix of 76 Staples 500 1 10