Table of Contents

Water wings maze

Water wings maze

Plates

A01 -	A02 (0,0)	A03 (3,1)	A04 (1,3)	A05 (4,4)	A06 -	A07 (0,0)	A08 (3,1)	A09 (1,3)	A10 (4,4)	A11 -	A12 -
B01 -	B02 (1,0)	B03 (4,1)	B04 (2,3)	B05 (5,0)	B06 -	B07 (1,0)	B08 (4,1)	B09 (2,3)	B10 (5,0)	B11 -	B12 -
C01 -	C02 (2,0)	C03 (0,2)	C04 (3,3)	C05 (5,1)	C06 -	C07 (2,0)	C08 (0,2)	C09 (3,3)	C10 (5,1)	C11 -	C12 -
D01 -	D02 (3,0)	D03 (1,2)	D04 (4,3)	D05 (5,2)	D06 -	D07 (3,0)	D08 (1,2)	D09 (4,3)	D10 (5,2)	D11 -	D12 -
E01 -	E02 (4,0)	E03 (2,2)	E04 (0,4)	E05 (5,3)	E06 -	E07 (4,0)	E08 (2,2)	E09 (0,4)	E10 (5,3)	E11 -	E12 -
F01 -	F02 (0,1)	F03 (3,2)	F04 (1,4)	F05 (5,4)	F06 -	F07 (0,1)	F08 (3,2)	F09 (1,4)	F10 (5,4)	F11 -	F12 -
G01 -	G02 (1,1)	G03 (4,2)	G04 (2,4)	G05 -	G06 -	G07 (1,1)	G08 (4,2)	G09 (2,4)	G10 -	G11 -	G12 -
H01 -	H02 (2,1)	H03 (0,3)	H04 (3,4)	H05 -	H06 -	H07 (2,1)	H08 (0,3)	H09 (3,4)	H10 -	H11 -	H12 -

The maze coordinates:

HY \ NX	0	1	2	3	4	5
4	E04 E09	F04 F09	G04 G09	H04 H09	A05 A10	F05 F10	4
3	H03 H08	A04 A09	B04 B09	C04 C09	D04 D09	E05 E10	3
2	C03 C08	D03 D08	E03 E08	F03 F08	G03 G08	D05 D10	2
1	F02 F07	G02 G07	H02 H07	A03 A08	B03 B08	C05 C10	1
0	A02 A07	B02 B07	C02 C07	D02 D07	E02 E07	B05 B10	0
HY / NX	0	1	2	3	4	5

Freezer Mix

In 4x 2mL-tubes:

Tube 2020.09.17-A: 48 strands A1-H6 plate 1/3 2mL@2mM
Tube 2020.09.17-B: 48 strands A7-H12 plate 1/3 2mL@2mM
Tube 2020.09.17-C: 48 strands A1-H6 plate 2/3 2mL@2mM
Tube 2020.09.17-D: 28 strands A7-D10 plate 2/3 2mL@2mM

48 Non-docking strands Freezer Mix (2mL@2mM)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
one 2mL tube of 48 non-docking strands				2000
H₂O
1 Non-docking strand	200000	48	2000

28 Non-docking strands Freezer Mix (2mL@2mM)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
one 2mL tube of 28 non-docking strands				2000
H₂O
1 Non-docking strand	200000	28	2000

In 2×30 2mL tubes:

Tube 2020.09.17-Δx:y (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM
Tube 2020.09.17-Δx:y+ (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM

1 docking strand Freezer Mix (2mL@5mM)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
one 5mL tube of 1 docking strand				2000
H₂O
1 docking strand	200000	1	5000

Fridge Mix

In 1x 2mL-tube:

Tube 2020.09.17-E: all 3×48+28 = 172 strands A1-H12@(plate 1/3)+A1-D10@(plate 2/3) 2mL@100nM

All 172 Non-docking strands Fridge Mix (2mL@100nM)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
one 2mL tube of all 172 non-docking strands				2000
H₂O
1 Non-docking strand freezer mix	2000	4	100

In 2×30 2mL tubes:

Tube 2020.09.17-δx:y (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@500uM
Tube 2020.09.17-δx:y+ (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@500uM

1 docking strand Fridge Mix (2mL@500uM)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
one 2mL-tube of 1 docking strand				2000
H₂O
1 docking strand freezer mix	5000	1	500

Maze Mix

In 500uL-tube:

Y\X	0	1	2	3	4	5
0	o	o	o	o	o	o
1	o	#	o	#	o	#
2	o	o	o	o	o	o
3	#	o	#	o	#	o
4	o	o	o	o	o	o

Y\X	0	1	2	3	4	5
0	#	o	#	o	#	o
1	o	o	o	o	o	o
2	o	#	o	#	o	#
3	o	o	o	o	o	o
4	#	o	#	o	#	o

Y\X	0	1	2	3	4	5
0	#	o	#	o	#	o
1	o	#	o	#	o	#
2	#	o	#	o	#	o
3	o	#	o	#	o	#
4	#	o	#	o	#	o

Y\X	0	1	2	3	4	5
0	o	#	o	#	o	#
1	#	o	#	o	#	o
2	o	#	o	#	o	#
3	#	o	#	o	#	o
4	o	#	o	#	o	#

Origami mix

In a 500uL tube:

Origami Mix (50uL)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
Origami Mix				50
H₂O
TAE Mg 10x	10	1	1
Fridge M13	10	1	1
Fridge Mix of all 172 non-docking staples	100	1	10
Fridge mix of each 30 docking staples	500	30	10