2021.01.19_m2if_cr11_rocket

Table of Contents

Origami: Rocket 2x layer (M2IF CR11 2021)

Origami: Rocket 2x layer (M2IF CR11 2021)

IDT plates

208 stapple strands @200μM each

Freezer mix

In 0.5mL tubes:

I'll make 6×32 = of those:
- 2021.01.20A: wells P1:A1-H4
- 2021.01.20B: wells P1:A5-H8
- 2021.01.20C: wells P1:A9-H12
- 2021.01.20D: wells P2:A1-H4
- 2021.01.20E: wells P2:A5-H8
- 2021.01.20F: wells P2:A9-H12

Freezer Mix of 32 Staples (400uL at 5000nM each)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
Volume				400
H₂O
staple	200000	32	5000

I'll make 1 of this:
- 2021.01.20G: wells P3:A1-H2

Freezer Mix of Staples (400uL at 5000nM each)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
Volume				400
H₂O
staple	200000		5000

Fridge mix

In 0.5mL tube:

2021.01.20H: All 208 staples 500uL@500nM

Fridge Mix of 6×32+16= Staples (500uL at 500nM)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
Volume				500
H₂O
Freezer Mix of 32 Staples (5000nM)	5000	6	500
Freezer Mix of 16 Staples (5000nM)	5000	1	500

M13 fridge tube

10nM p7249 M13mp18 fridge tube 500mL
Product	Concentration (mM)	Number	Target concentration (mM)	Volume (uL)	Vol*nb
Volume				500
H₂O
100nM Tilibit p7249 M13mp48	100	1	10

Store the rest of the Tilibit p7249 M13mp18 2ml 100nM in Freezer (-20°C)

Origami mix

In a 0.5mL tube:

2021.01.20I: Rocket origami mix 50uL@1nM

Origami Mix (500uL)
Product	Concentration (nM)	Number	Target concentration (nM)	Volume (uL)	Vol*nb
Volume				50
H₂O
TAE Mg 10x	10	1	1
Fridge M13	10	1	1
Fridge Mix of 208 Staples	500	1	10