Table of Contents

Origami: Space Invader

Freezer mix

In 0.5mL tubes:

  • I'll make 9×16 = of those:
Freezer Mix of 16 Staples (300uL at 2500nM each)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Freezer Mix of 16 Staples (2500nM) 300
H2O
staple 50000 16 2500
  • I'll make 1 of this:
Freezer Mix of 12 Staples (300uL at 2500nM each)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Freezer Mix of 12 Staples (5000nM) 300
H2O
staple 50000 12 2500

Fridge mix

In 0.5mL tubes:

  • I'll make 1 of these:
Fridge Mix of 80 Staples (500uL at 500nM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Fridge Mix of 80 Staples (1000nM) 500
H2O
Freezer Mix of 16 Staples (2500nM) 2500 5 500
  • I'll make 1 of these:
Fridge Mix of 76 Staples (500uL at 500nM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Fridge Mix of Staples (1000nM) 500
H2O
Freezer Mix of 16 Staples (2500nM) 2500 4 500
Freezer Mix of 12 Staples (2500nM) 2500 1 500

M13 fridge tube

10nM p7249 M13mp18 fridge tube 500mL
Product Concentration (mM) Number Target concentration (mM) Volume (uL) Vol*nb
10nM p7249 M13mp18 500
H2O
100nM Tilibit p7249 M13mp48 100 1 10

Store the rest of the Tilibit p7249 M13mp18 2ml 100nM in Freezer (-20°C)

Origami mix

In a 0.5mL tube:

Origami Mix (500uL)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Origami Mix 500
H2O
TAE Mg 10x 10 1 1
Fridge M13 10 1 1
Fridge Mix of 80 Staples 500 1 10
Fridge Mix of 76 Staples 500 1 10