Experiment 2020.03.10
2020.10.03: sample preparation
Recooked 6 mixes:
- 2020.03.10-A 2xlayer origami SE:noHP NW:hp @50nM
- 2020.03.10-B 2xlayer origami SE:noHP NW:hp @1nM
- 2020.03.10-C 2xlayer origami All:hp @1nM
- 2020.03.10-D Space invaders origami Mix1 @5nM
- 2020.03.10-E Space invaders origami Mix2 @5nM
- 2020.03.10-F Space invaders origami Marcus @1nM
Space invader mix 50uL@5nM:
- TAE 10x → 1x: 5uL
- M13 10nM → 5nM: 25uL
- All staples 200nM → 50nM: 12.5uL
- H20: 7.5uL
Temperature:
- 90°C 20min
- 90°C to 65°C -1°C/min
- 65°C to 45°C -1°C/h
- 25°C forever
AFM
Mica:
- 2020.03.10-A 20uL
- 2020.03.10-B 50uL
- 2020.03.10-E 50uL
Rincing:
- 5min rest
- Na 1x 60uL 5min
- (3x) Mg 1x 60uL 5min