Table of Contents

Water wings maze

Plates

A01
- 		A02
(0,0) 		A03
(3,1) 		A04
(1,3) 		A05
(4,4) 		A06
- 		A07
(0,0) 		A08
(3,1) 		A09
(1,3) 		A10
(4,4) 		A11
- 		A12
-
B01
- 		B02
(1,0) 		B03
(4,1) 		B04
(2,3) 		B05
(5,0) 		B06
- 		B07
(1,0) 		B08
(4,1) 		B09
(2,3) 		B10
(5,0) 		B11
- 		B12
-
C01
- 		C02
(2,0) 		C03
(0,2) 		C04
(3,3) 		C05
(5,1) 		C06
- 		C07
(2,0) 		C08
(0,2) 		C09
(3,3) 		C10
(5,1) 		C11
- 		C12
-
D01
- 		D02
(3,0) 		D03
(1,2) 		D04
(4,3) 		D05
(5,2) 		D06
- 		D07
(3,0) 		D08
(1,2) 		D09
(4,3) 		D10
(5,2) 		D11
- 		D12
-
E01
- 		E02
(4,0) 		E03
(2,2) 		E04
(0,4) 		E05
(5,3) 		E06
- 		E07
(4,0) 		E08
(2,2) 		E09
(0,4) 		E10
(5,3) 		E11
- 		E12
-
F01
- 		F02
(0,1) 		F03
(3,2) 		F04
(1,4) 		F05
(5,4) 		F06
- 		F07
(0,1) 		F08
(3,2) 		F09
(1,4) 		F10
(5,4) 		F11
- 		F12
-
G01
- 		G02
(1,1) 		G03
(4,2) 		G04
(2,4) 		G05
- 		G06
- 		G07
(1,1) 		G08
(4,2) 		G09
(2,4) 		G10
- 		G11
- 		G12
-
H01
- 		H02
(2,1) 		H03
(0,3) 		H04
(3,4) 		H05
- 		H06
- 		H07
(2,1) 		H08
(0,3) 		H09
(3,4) 		H10
- 		H11
- 		H12
-

The maze coordinates:

HY \ NX 0 1 2 3 4 5
4 E04
E09 		F04
F09 		G04
G09 		H04
H09 		A05
A10 		F05
F10 		4
3 H03
H08 		A04
A09 		B04
B09 		C04
C09 		D04
D09 		E05
E10 		3
2 C03
C08 		D03
D08 		E03
E08 		F03
F08 		G03
G08 		D05
D10 		2
1 F02
F07 		G02
G07 		H02
H07 		A03
A08 		B03
B08 		C05
C10 		1
0 A02
A07 		B02
B07 		C02
C07 		D02
D07 		E02
E07 		B05
B10 		0
HY / NX 0 1 2 3 4 5

Freezer Mix

In 4x 2mL-tubes:

  • Tube 2020.09.17-A: 48 strands A1-H6 plate 1/3 2mL@2mM
  • Tube 2020.09.17-B: 48 strands A7-H12 plate 1/3 2mL@2mM
  • Tube 2020.09.17-C: 48 strands A1-H6 plate 2/3 2mL@2mM
  • Tube 2020.09.17-D: 28 strands A7-D10 plate 2/3 2mL@2mM
48 Non-docking strands Freezer Mix (2mL@2mM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL tube of 48 non-docking strands 2000
H2O
1 Non-docking strand 200000 48 2000
28 Non-docking strands Freezer Mix (2mL@2mM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL tube of 28 non-docking strands 2000
H2O
1 Non-docking strand 200000 28 2000

In 2×30 2mL tubes:

  • Tube 2020.09.17-Δx:y (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM
  • Tube 2020.09.17-Δx:y+ (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM
1 docking strand Freezer Mix (2mL@5mM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 5mL tube of 1 docking strand 2000
H2O
1 docking strand 200000 1 5000

Fridge Mix

In 1x 2mL-tube:

  • Tube 2020.09.17-E: all 3×48+28 = 172 strands A1-H12@(plate 1/3)+A1-D10@(plate 2/3) 2mL@100nM
All 172 Non-docking strands Fridge Mix (2mL@100nM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL tube of all 172 non-docking strands 2000
H2O
1 Non-docking strand freezer mix 2000 4 100

In 2×30 2mL tubes:

  • Tube 2020.09.17-δx:y (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@500uM
  • Tube 2020.09.17-δx:y+ (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@500uM
1 docking strand Fridge Mix (2mL@500uM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL-tube of 1 docking strand 2000
H2O
1 docking strand freezer mix 5000 1 500

Maze Mix

In 500uL-tube:

Y\X 0 1 2 3 4 5
0 o o o o o o
1 o # o # o #
2 o o o o o o
3 # o # o # o
4 o o o o o o
Y\X 0 1 2 3 4 5
0 # o # o # o
1 o o o o o o
2 o # o # o #
3 o o o o o o
4 # o # o # o
Y\X 0 1 2 3 4 5
0 # o # o # o
1 o # o # o #
2 # o # o # o
3 o # o # o #
4 # o # o # o
Y\X 0 1 2 3 4 5
0 o # o # o #
1 # o # o # o
2 o # o # o #
3 # o # o # o
4 o # o # o #

Origami mix

In a 500uL tube:

Origami Mix (50uL)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Origami Mix 50
H2O
TAE Mg 10x 10 1 1
Fridge M13 10 1 1
Fridge Mix of all 172 non-docking staples 100 1 10
Fridge mix of each 30 docking staples 500 30 10