Table of Contents
Origami: Rocket 2x layer (M2IF CR11 2021)
IDT plates
208 stapple strands @200μM each
Freezer mix
In 0.5mL tubes:
- I'll make 6×32 = of those:
- 2021.01.20A: wells P1:A1-H4
- 2021.01.20B: wells P1:A5-H8
- 2021.01.20C: wells P1:A9-H12
- 2021.01.20D: wells P2:A1-H4
- 2021.01.20E: wells P2:A5-H8
- 2021.01.20F: wells P2:A9-H12
| Freezer Mix of 32 Staples (400uL at 5000nM each) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| Volume | 400 | ||||
| H2O | |||||
| staple | 200000 | 32 | 5000 | ||
- I'll make 1 of this:
- 2021.01.20G: wells P3:A1-H2
| Freezer Mix of Staples (400uL at 5000nM each) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| Volume | 400 | ||||
| H2O | |||||
| staple | 200000 | 5000 | |||
Fridge mix
In 0.5mL tube:
- 2021.01.20H: All 208 staples 500uL@500nM
| Fridge Mix of 6×32+16= Staples (500uL at 500nM) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| Volume | 500 | ||||
| H2O | |||||
| Freezer Mix of 32 Staples (5000nM) | 5000 | 6 | 500 | ||
| Freezer Mix of 16 Staples (5000nM) | 5000 | 1 | 500 | ||
M13 fridge tube
| 10nM p7249 M13mp18 fridge tube 500mL | |||||
|---|---|---|---|---|---|
| Product | Concentration (mM) | Number | Target concentration (mM) | Volume (uL) | Vol*nb |
| Volume | 500 | ||||
| H2O | |||||
| 100nM Tilibit p7249 M13mp48 | 100 | 1 | 10 | ||
Store the rest of the Tilibit p7249 M13mp18 2ml 100nM in Freezer (-20°C)
Origami mix
In a 0.5mL tube:
- 2021.01.20I: Rocket origami mix 50uL@1nM
| Origami Mix (500uL) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| Volume | 50 | ||||
| H2O | |||||
| TAE Mg 10x | 10 | 1 | 1 | ||
| Fridge M13 | 10 | 1 | 1 | ||
| Fridge Mix of 208 Staples | 500 | 1 | 10 | ||