PM2 2021.03.04 WaterWings cooking test
goal : Cook the water wings and observe the docking blob on the AFM.
Cooking
Master Mix
docking staples design :
Vtot = 60 uL
| product | concentration | target concentration | volume |
| H20 | 10uL | ||
| TAE Mg 10X | 10 nM | 1 nM | 10 uL |
| Fridge M13 | 10 nM | 1 nM | 10 uL |
| Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 10uL |
| for each 30 docking staples | 500 nM | 10nM | 2 uL |
tube WW A : 30uL Master Mix tube WW B : 30uL Master Mix
5 min 65deg 65 → 55 : 15 min per degree 55 → 40 : 140 min per degree hold at 25 deg
TODO CHECK THE TEMPERATURE CURVE
Afm
sample 1 - Na 2 times, TAE 2 times WW A 25uL Na 20uL 3 min Na 20uL 3 min TAE 1X 20uL 3 min TAE 1X 20uL 3 min TAE 1X 60uL
images on the AFM PC
sample 2 - Na, TAE WW B 25uL Na 20uL 3 min TAE 1X 20uL 3 min TAE 1X 60uL
forgot to save the image ?
sample 3 - direct WW A 25uL Na 20uL 3 min
images on the AFM PC
Conclusion
Sample 3 has a lot more of origamis compared to sample 1 ( about several times more) → rising loose many origamis
Some stacking between the water wings : two origamis stuck together