Last update: 26.10.2011
The UNIO protocol (see Research) for fully automated protein NMR structure determination directly operating on NMR spectra has been successfully applied to several de novo structure determination projects ( > 30 de novo protein structure determination projects initiated within Proteomics Structure Initiatives with a molecular weight ranging from 8 to 22 kDa).
All of the 3D protein NMR structures have been determined within approximately two weeks including the acquisition of the 6 NMR spectra required (3 APSY-type and 3 NOESY-type NMR experiments, please note that conventional triple-resonance NMR data can alternatively be used).
Currently (Oct 2011), 30 of them determined with the highly/fully automated UNIO protocol - including backbone assignment, side-chain assignment and NOE assignment - are available in the Protein Data Bank (PDB).
The UNIO protocol was released in Jan. 2008 to the NMR community.
Collage of PDB entries of NMR structures determined by the highly/fully automated UNIO protocol.
(Only PDB entries have been selected that explicitly list MATCH, ATNOS/ASCAN, ATNOS/CANDID or UNIO as software used.)
Number of PDB structures: 30 (Oct 17, 2011)
Molecular weight distribution of PDB entries of NMR structures determined by UNIO.
(Only PDB entries have been selected that explicitly list MATCH, ATNOS/ASCAN, ATNOS/CANDID or UNIO as software used.)
