Differences
This shows you the differences between two versions of the page.
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2019.11.25_space_invaders [2019/11/25 15:00] Nicolas Schabanel [M13 fridge tube] |
2019.11.25_space_invaders [2021/03/05 15:29] |
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| - | ===== Origami: Space Invaders ===== | ||
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| - | ==== Freezer mix ==== | ||
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| - | In 0.5mL tubes: | ||
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| - | * I'll make **9x16 = ~~=9*16~~** of those: | ||
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| - | ^ //Freezer Mix of 16 Staples (300uL at 2500nM each) // ^^^^^^ | ||
| - | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Freezer Mix of 16 Staples (2500nM) ^^^^ 300 ^^ | ||
| - | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ staple | 50000 | 16 | 2500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
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| - | * I'll make 1 of this: | ||
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| - | ^ //Freezer Mix of 12 Staples (300uL at 2500nM each) // ^^^^^^ | ||
| - | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Freezer Mix of 12 Staples (5000nM) ^^^^ 300 ^^ | ||
| - | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ staple | 50000 | 12 | 2500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
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| - | ==== Fridge mix ==== | ||
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| - | In 0.5mL tubes: | ||
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| - | * I'll make 1 of these: | ||
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| - | ^ //Fridge Mix of 80 Staples (500uL at 500nM) // ^^^^^^ | ||
| - | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Fridge Mix of 80 Staples (1000nM) ^^^^ 500 ^^ | ||
| - | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ Freezer Mix of 16 Staples (2500nM) | 2500 | 5 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
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| - | * I'll make 1 of these: | ||
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| - | ^ //Fridge Mix of 76 Staples (500uL at 500nM) // ^^^^^^ | ||
| - | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Fridge Mix of ~~=4*16+12~~ Staples (1000nM) ^^^^ 500 ^^ | ||
| - | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ Freezer Mix of 16 Staples (2500nM) | 2500 | 4 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| - | ^ Freezer Mix of 12 Staples (2500nM) | 2500 | 1 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
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| - | ==== M13 fridge tube ==== | ||
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| - | ^ //10nM p7249 M13mp18 fridge tube 500mL// ^^^^^^ | ||
| - | ^ Product ^ Concentration (mM) ^ Number ^ Target concentration (mM) ^ Volume (uL) ^ <color gray>Vol*nb</color> ^ | ||
| - | ^ 10nM p7249 M13mp18 ^^^^ 500 ^^ | ||
| - | ^ H<sub>2</sub>O ^^^^ **~~=r2c1 - sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ 100nM Tilibit p7249 M13mp48 | 100 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
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| - | Store the rest of the Tilibit p7249 M13mp18 2ml 100nM in Freezer (-20°C) | ||
| - | ==== Origami mix ==== | ||
| - | In a 0.5mL tube: | ||
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| - | ^ //Origami Mix (500uL) // ^^^^^^ | ||
| - | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Origami Mix ^^^^ 500 ^^ | ||
| - | ^ H<sub>2</sub>O ^^^^ ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ TAE Mg 10x | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| - | ^ Fridge M13 | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| - | ^ Fridge Mix of 80 Staples | 500 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| - | ^ Fridge Mix of 76 Staples | 500 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||