This is an old revision of the document!
Water wings maze
Plates
Freezer Mix
In 4x 2mL-tubes:
- Tube 2020.09.17-A: 48 strands A1-H6 plate 1/3 2mL@2mM
- Tube 2020.09.17-B: 48 strands A7-H12 plate 1/3 2mL@2mM
- Tube 2020.09.17-C: 48 strands A1-H6 plate 2/3 2mL@2mM
- Tube 2020.09.17-D: 28 strands A7-D10 plate 2/3 2mL@2mM
| 48 Non-docking strands Freezer Mix (2mL@2mM) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| one 2mL tube of 48 non-docking strands | 2000 | ||||
| H2O | |||||
| 1 Non-docking strand | 200000 | 48 | 2000 | ||
| 28 Non-docking strands Freezer Mix (2mL@2mM) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| one 2mL tube of 28 non-docking strands | 2000 | ||||
| H2O | |||||
| 1 Non-docking strand | 200000 | 28 | 2000 | ||
In 2×30 2mL tubes:
- Tube 2020.09.17-xy(x,y) (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM
- Tube 2020.09.17-XY(x,y) (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM
| 1 docking strand Freezer Mix (2mL@5mM) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| one 5mL tube of 1 docking strand | 2000 | ||||
| H2O | |||||
| 1 docking strand | 200000 | 1 | 5000 | ||
Fridge Mix
Origami mix
In a 0.5mL tube:
| Origami Mix (500uL) | |||||
|---|---|---|---|---|---|
| Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
| Origami Mix | 500 | ||||
| H2O | |||||
| TAE Mg 10x | 10 | 1 | 1 | ||
| Fridge M13 | 10 | 1 | 1 | ||
| Fridge Mix of 170 non-HPable Staples | 1000 | 1 | 10 | ||
| Fridge of HPable Staples | 1000 | 42 | 10 | ||