This is an old revision of the document!
Water wings maze
Plates
Freezer Mix
In 4x 2mL-tubes:
- Tube 2020.09.17-A: 48 strands A1-H6 plate 1/3 2mL@2mM
- Tube 2020.09.17-B: 48 strands A7-H12 plate 1/3 2mL@2mM
- Tube 2020.09.17-C: 48 strands A1-H6 plate 2/3 2mL@2mM
- Tube 2020.09.17-D: 28 strands A7-D10 plate 2/3 2mL@2mM
48 Non-docking strands Freezer Mix (2mL@2mM) | |||||
---|---|---|---|---|---|
Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
one 2mL tube of 48 non-docking strands | 2000 | ||||
H2O | |||||
1 Non-docking strand | 200000 | 48 | 2000 |
28 Non-docking strands Freezer Mix (2mL@2mM) | |||||
---|---|---|---|---|---|
Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
one 2mL tube of 28 non-docking strands | 2000 | ||||
H2O | |||||
1 Non-docking strand | 200000 | 28 | 2000 |
In 2×30 2mL tubes:
- Tube 2020.09.17-xy(x,y) (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM
- Tube 2020.09.17-XY(x,y) (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM
1 docking strand Freezer Mix (2mL@5mM) | |||||
---|---|---|---|---|---|
Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
one 5mL tube of 1 docking strand | 2000 | ||||
H2O | |||||
1 docking strand | 200000 | 1 | 5000 |
Fridge Mix
In 1x 2mL-tube:
- Tube 2020.09.17-E: all 3×48+28 = 172 strands A1-H12@(plate 1/3)+A1-D10@(plate 2/3) 2mL@100nM
All 172 Non-docking strands Fridge Mix (2mL@100nM) | |||||
---|---|---|---|---|---|
Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
one 2mL tube of all 172 non-docking strands | 2000 | ||||
H2O | |||||
1 Non-docking strand freezer mix | 2000 | 4 | 100 |
In 2×30 2mL tubes:
- Tube 2020.09.17-xy(x,y) (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@500uM
- Tube 2020.09.17-XY(x,y) (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@500uM
1 docking strand Fridge Mix (2mL@500uM) | |||||
---|---|---|---|---|---|
Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
one 2mL-tube of 1 docking strand | 2000 | ||||
H2O | |||||
1 docking strand freezer mix | 5000 | 1 | 500 |
Origami mix
In a 500uL tube:
Origami Mix (50uL) | |||||
---|---|---|---|---|---|
Product | Concentration (nM) | Number | Target concentration (nM) | Volume (uL) | Vol*nb |
Origami Mix | 50 | ||||
H2O | |||||
TAE Mg 10x | 10 | 1 | 1 | ||
Fridge M13 | 10 | 1 | 1 | ||
Fridge Mix of all 172 non-docking staples | 100 | 1 | 10 | ||
Fridge mix of each 30 docking staples | 500 | 30 | 10 |