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Water wings maze

Plates

A01
- 		A02
(0,0) 		A03
(3,1) 		A04
(1,3) 		A05
(4,4) 		A06
- 		A07
(0,0) 		A08
(3,1) 		A09
(1,3) 		A10
(4,4) 		A11
- 		A12
-
B01
- 		B02
(1,0) 		B03
(4,1) 		B04
(2,3) 		B05
(5,0) 		B06
- 		B07
(1,0) 		B08
(4,1) 		B09
(2,3) 		B10
(5,0) 		B11
- 		B12
-
C01
- 		C02
(2,0) 		C03
(0,2) 		C04
(3,3) 		C05
(5,1) 		C06
- 		C07
(2,0) 		C08
(0,2) 		C09
(3,3) 		C10
(5,1) 		C11
- 		C12
-
D01
- 		D02
(3,0) 		D03
(1,2) 		D04
(4,3) 		D05
(5,2) 		D06
- 		D07
(3,0) 		D08
(1,2) 		D09
(4,3) 		D10
(5,2) 		D11
- 		D12
-
E01
- 		E02
(4,0) 		E03
(2,2) 		E04
(0,4) 		E05
(5,3) 		E06
- 		E07
(4,0) 		E08
(2,2) 		E09
(0,4) 		E10
(5,3) 		E11
- 		E12
-
F01
- 		F02
(0,1) 		F03
(3,2) 		F04
(1,4) 		F05
(5,4) 		F06
- 		F07
(0,1) 		F08
(3,2) 		F09
(1,4) 		F10
(5,4) 		F11
- 		F12
-
G01
- 		G02
(1,1) 		G03
(4,2) 		G04
(2,4) 		G05
- 		G06
- 		G07
(1,1) 		G08
(4,2) 		G09
(2,4) 		G10
- 		G11
- 		G12
-
H01
- 		H02
(2,1) 		H03
(0,3) 		H04
(3,4) 		H05
- 		H06
- 		H07
(2,1) 		H08
(0,3) 		H09
(3,4) 		H10
- 		H11
- 		H12
-

The square origami: West and East are on the top layer, North and South on the bottom layer

N1
D11 		N2
A04 		N3
E11 		N4
B04 		N5
A12 		N6
C04 		N7
B12 		N8
D04 		N9
C12 		N10
E04
W1
A05 		E1
C10
W2
A01 		E2
A03
W3
B05 		E3
D10
W4
B01 		E4
B03
W5
C05 		E5
E10
W6
C01 		E6
C03
W7
D05 		E7
A11
W8
D01 		E8
D03
W9
E05 		E9
B11
W10
E01 		E10
E03
E11
C11
S1
A06 		S2
A02 		S3
B06 		S4
B02 		S5
C06 		S6
C02 		S7
D06 		S8
D02 		S9
E06 		S10
E02 		S11
A07

Freezer Mix

In 4x 2mL-tubes:

  • Tube 2020.09.17-A: 48 strands A1-H6 plate 1/3 2mL@2mM
  • Tube 2020.09.17-B: 48 strands A7-H12 plate 1/3 2mL@2mM
  • Tube 2020.09.17-C: 48 strands A1-H6 plate 2/3 2mL@2mM
  • Tube 2020.09.17-D: 28 strands A7-D10 plate 2/3 2mL@2mM
48 Non-docking strands Freezer Mix (2mL@2mM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL tube of 48 non-docking strands 2000
H2O
1 Non-docking strand 200000 48 2000
28 Non-docking strands Freezer Mix (2mL@2mM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL tube of 28 non-docking strands 2000
H2O
1 Non-docking strand 200000 28 2000

In 2×30 2mL tubes:

  • Tube 2020.09.17-xy(x,y) (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM
  • Tube 2020.09.17-XY(x,y) (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM
1 docking strand Freezer Mix (2mL@5mM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 5mL tube of 1 docking strand 2000
H2O
1 docking strand 200000 1 5000

Fridge Mix

In 1x 2mL-tube:

  • Tube 2020.09.17-E: all 3×48+28 = 172 strands A1-H12@(plate 1/3)+A1-D10@(plate 2/3) 2mL@100nM
All 172 Non-docking strands Fridge Mix (2mL@100nM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL tube of all 172 non-docking strands 2000
H2O
1 Non-docking strand freezer mix 2000 4 100

In 2×30 2mL tubes:

  • Tube 2020.09.17-xy(x,y) (0,0)≤(x,y)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@500uM
  • Tube 2020.09.17-XY(x,y) (0,0)≤(x,y)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@500uM
1 docking strand Fridge Mix (2mL@500uM)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
one 2mL-tube of 1 docking strand 2000
H2O
1 docking strand freezer mix 5000 1 500

Origami mix

In a 500uL tube:

Origami Mix (50uL)
Product Concentration (nM) Number Target concentration (nM) Volume (uL) Vol*nb
Origami Mix 50
H2O
TAE Mg 10x 10 1 1
Fridge M13 10 1 1
Fridge Mix of all 172 non-docking staples 100 1 10
Fridge mix of each 30 docking staples 500 30 10