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2021.03.05_pm3_water_wings_melt [2021/03/05 09:19] Pierre Marcus created |
2021.03.05_pm3_water_wings_melt [2021/03/05 15:29] (current) |
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| **Goal : do a melt curve of the WW origami** | **Goal : do a melt curve of the WW origami** | ||
| - | Vtot = 60 uL | + | Master Mix : |
| + | Vtot =200 uL | ||
| | product | concentration | target concentration | volume | | | product | concentration | target concentration | volume | | ||
| - | | H20 | | | 2uL| | + | | TAE Mg 10X | 10 nM | 1 nM | 20 uL | |
| - | | TAE Mg 10X | 10 nM | 1 nM | 10 uL | | + | | Fridge M13 | 10 nM | 1 nM | 20 uL | |
| - | | Fridge M13 | 10 nM | 1 nM | 10 uL | | + | | Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 20uL | |
| - | | Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 10uL | | + | | for each 30 docking staples | 500 nM | 10nM | 4 uL | |
| - | | for each 30 docking staples | 500 nM | 10nM | 1 uL | | + | | Sybr 20X | | | 10 uL| |
| - | | evagreen 20X | | | 3 uL| | + | | H20 | | | 10uL| |
| + | |||
| + | 8 pcr tube of 20uL master mix | ||
| + | |||
| + | melt curve | ||
| + | total time 11h | ||