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021.03.05_pm3_water_wings_melt
Goal : do a melt curve of the WW origami
Vtot = 30 uL
product | concentration | target concentration | volume |
H20 | 1uL | ||
TAE Mg 10X | 10 nM | 1 nM | 5 uL |
Fridge M13 | 10 nM | 1 nM | 5 uL |
Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 5uL |
for each 30 docking staples | 500 nM | 10nM | 1 uL |
evagreen 20X | 1.5 uL |