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2021.03.05_pm3_water_wings_melt [2021/03/05 09:20] Pierre Marcus |
2021.03.05_pm3_water_wings_melt [2021/03/05 15:29] (current) |
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**Goal : do a melt curve of the WW origami** | **Goal : do a melt curve of the WW origami** | ||
- | Vtot = 30 uL | + | Master Mix : |
+ | Vtot =200 uL | ||
| product | concentration | target concentration | volume | | | product | concentration | target concentration | volume | | ||
- | | H20 | | | 1uL| | + | | TAE Mg 10X | 10 nM | 1 nM | 20 uL | |
- | | TAE Mg 10X | 10 nM | 1 nM | 5 uL | | + | | Fridge M13 | 10 nM | 1 nM | 20 uL | |
- | | Fridge M13 | 10 nM | 1 nM | 5 uL | | + | | Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 20uL | |
- | | Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 5uL | | + | | for each 30 docking staples | 500 nM | 10nM | 4 uL | |
- | | for each 30 docking staples | 500 nM | 10nM | 1 uL | | + | | Sybr 20X | | | 10 uL| |
- | | evagreen 20X | | | 1.5 uL| | + | | H20 | | | 10uL| |
+ | |||
+ | 8 pcr tube of 20uL master mix | ||
+ | |||
+ | melt curve | ||
+ | total time 11h |