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2021.03.05_pm3_water_wings_melt [2021/03/05 13:25] Pierre Marcus |
2021.03.05_pm3_water_wings_melt [2021/03/05 15:29] (current) |
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| Line 4: | Line 4: | ||
| **Goal : do a melt curve of the WW origami** | **Goal : do a melt curve of the WW origami** | ||
| + | Master Mix : | ||
| Vtot =200 uL | Vtot =200 uL | ||
| | product | concentration | target concentration | volume | | | product | concentration | target concentration | volume | | ||
| Line 10: | Line 11: | ||
| | Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 20uL | | | Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 20uL | | ||
| | for each 30 docking staples | 500 nM | 10nM | 4 uL | | | for each 30 docking staples | 500 nM | 10nM | 4 uL | | ||
| - | | Sybr 20X | | | 1.5 uL| | + | | Sybr 20X | | | 10 uL| |
| | H20 | | | 10uL| | | H20 | | | 10uL| | ||
| + | |||
| + | 8 pcr tube of 20uL master mix | ||
| + | |||
| + | melt curve | ||
| + | total time 11h | ||