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| Both sides previous revision Previous revision Next revision | Previous revision | ||
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2021.03.05_pm3_water_wings_melt [2021/03/05 13:25] Pierre Marcus |
2021.03.05_pm3_water_wings_melt [2021/03/05 15:29] (current) |
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| Line 4: | Line 4: | ||
| **Goal : do a melt curve of the WW origami** | **Goal : do a melt curve of the WW origami** | ||
| + | Master Mix : | ||
| Vtot =200 uL | Vtot =200 uL | ||
| | product | concentration | target concentration | volume | | | product | concentration | target concentration | volume | | ||
| Line 13: | Line 14: | ||
| | H20 | | | 10uL| | | H20 | | | 10uL| | ||
| + | |||
| + | 8 pcr tube of 20uL master mix | ||
| + | |||
| + | melt curve | ||
| + | total time 11h | ||