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TODO WW new cooking
use the same protocol as in “How We Make DNA Origami” “the reaction mixtures were first incubated at 65 8C for 15 min and then cooled from 60 to 44 8C in steps of 1 deg per hour”
Master mix : Vtot = 60 uL
product | concentration | target concentration | volume |
H20 | 10uL | ||
TAE Mg 10X | 10 nM | 1 nM | 10 uL |
Fridge M13 | 10 nM | 1 nM | 10 uL |
Fridge Mix of all 172 non docking staples | 100 nM | 10 nM | 10uL |
for each 30 docking staples | 500 nM | 10nM | 2 uL |