Differences

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--- 2019.11.25_space_invaders [2019/11/25 14:41]
Nicolas Schabanel created
+++ 2019.11.25_space_invaders [2021/03/05 15:29] (current)
@@ Line 1: / Line 1: @@
-===== Origami: Space Invaders =====
+===== Origami: Space Invader =====
 ==== Freezer mix ====
@@ Line 5: / Line 5: @@
 In 0.5mL tubes:
-  * I'll make **~~=156/16~~** of those:
+  * I'll make **9x16 = ~~=9*16~~** of those:
-^  //Freezer Mix of 24 Staples (300uL at 5000nM each) //  ^^^^^^
+^  //Freezer Mix of 16 Staples (300uL at 2500nM each) //  ^^^^^^
 ^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
-^  Freezer Mix of Staples (5000nM) ^^^^  300 ^^
+^  Freezer Mix of 16 Staples (2500nM) ^^^^  300 ^^
 ^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
 ^ staple  |  50000 |  16 |  2500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+  * I'll make 1 of this:
+^  //Freezer Mix of 12 Staples (300uL at 2500nM each) //  ^^^^^^
+^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
+^  Freezer Mix of 12 Staples (5000nM) ^^^^  300 ^^
+^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ staple  |  50000 |  12 |  2500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+==== Fridge mix ====
+In 0.5mL tubes:
+  * I'll make 1 of these:
+^  //Fridge Mix of 80 Staples (500uL at 500nM) //  ^^^^^^
+^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
+^  Fridge Mix of 80 Staples (1000nM) ^^^^  500 ^^
+^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ Freezer Mix of 16 Staples (2500nM)  |  2500 |  5 |  500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+  * I'll make 1 of these:
+^  //Fridge Mix of 76 Staples (500uL at 500nM) //  ^^^^^^
+^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
+^  Fridge Mix of ~~=4*16+12~~ Staples (1000nM) ^^^^  500 ^^
+^  H<sub>2</sub>O ^^^^  **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ Freezer Mix of 16 Staples (2500nM)  |  2500 |  4 |  500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+^ Freezer Mix of 12 Staples (2500nM)  |  2500 |  1 |  500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+==== M13 fridge tube ====
+^  //10nM p7249 M13mp18 fridge tube 500mL//  ^^^^^^
+^  Product  ^ Concentration (mM)  ^  Number  ^  Target concentration (mM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color> ^
+^  10nM p7249 M13mp18 ^^^^  500 ^^
+^  H<sub>2</sub>O ^^^^  **~~=r2c1 - sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ 100nM Tilibit p7249 M13mp48  |  100 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+Store the rest of the Tilibit p7249 M13mp18 2ml 100nM in Freezer (-20°C)
+==== Origami mix ====
+In a 0.5mL tube:
+^  //Origami Mix (500uL) //  ^^^^^^
+^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
+^  Origami Mix ^^^^  500 ^^
+^  H<sub>2</sub>O ^^^^  ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ TAE Mg 10x  |  10 |  1 |  1 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+^ Fridge M13  |  10 |  1 |  1 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+^ Fridge Mix of 80 Staples  |  500 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+^ Fridge Mix of 76 Staples  |  500 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |