Differences

This shows you the differences between two versions of the page.

--- 2020.09.14_water_wings_maze [2020/09/14 14:53]
Nicolas Schabanel [Freezer Mix]
+++ 2020.09.14_water_wings_maze [2021/03/05 15:29] (current)
@@ Line 2: / Line 2: @@
 ==== Plates ====
+| //A01//\\ - | //A02//\\ (0,0) | //A03//\\ (3,1) | //A04//\\ (1,3) | //A05//\\ (4,4) | //A06//\\ - | //A07//\\ (0,0) | //A08//\\ (3,1) | //A09//\\ (1,3) | //A10//\\ (4,4) | //A11//\\ - | //A12//\\ - |
+| //B01//\\ - | //B02//\\ (1,0) | //B03//\\ (4,1) | //B04//\\ (2,3) | //B05//\\ (5,0) | //B06//\\ - | //B07//\\ (1,0) | //B08//\\ (4,1) | //B09//\\ (2,3) | //B10//\\ (5,0)  | //B11//\\ - | //B12//\\ - |
+| //C01//\\ -  | //C02//\\ (2,0) | //C03//\\ (0,2) | //C04//\\ (3,3) | //C05//\\ (5,1) | //C06//\\ - | //C07//\\ (2,0) | //C08//\\ (0,2) | //C09//\\ (3,3) | //C10//\\ (5,1) | //C11//\\ - | //C12//\\ -|
+| //D01//\\ - | //D02//\\ (3,0) | //D03//\\ (1,2) | //D04//\\ (4,3) | //D05//\\ (5,2) | //D06//\\ - | //D07//\\ (3,0) | //D08//\\ (1,2) | //D09//\\ (4,3) | //D10//\\ (5,2) | //D11//\\ - | //D12//\\ -|
+| //E01//\\ - | //E02//\\ (4,0) | //E03//\\ (2,2) | //E04//\\ (0,4) | //E05//\\ (5,3) | //E06//\\ - | //E07//\\ (4,0) | //E08//\\ (2,2) | //E09//\\ (0,4) | //E10//\\ (5,3) | //E11//\\ - | //E12//\\ -|
+| //F01//\\ - | //F02//\\ (0,1) | //F03//\\ (3,2) | //F04//\\ (1,4) | //F05//\\ (5,4) | //F06//\\ - | //F07//\\ (0,1) | //F08//\\ (3,2) | //F09//\\ (1,4) | //F10//\\ (5,4) | //F11//\\ - | //F12//\\ -|
+| //G01//\\ - | //G02//\\ (1,1) | //G03//\\ (4,2) | //G04//\\ (2,4) | //G05//\\ - | //G06//\\ - | //G07//\\ (1,1) | //G08//\\ (4,2) | //G09//\\ (2,4) | //G10//\\ - | //G11//\\ - | //G12//\\ -|
+| //H01//\\ - | //H02//\\ (2,1) | //H03//\\ (0,3) | //H04//\\ (3,4) | //H05//\\ - | //H06//\\ - | //H07//\\ (2,1) | //H08//\\ (0,3) | //H09//\\ (3,4) | //H10//\\ - | //H11//\\ - | //H12//\\ -|
+The maze coordinates:
+| HY \ NX | 0     | 1         | 2         | 3         | 4         |         5 | |
+| 4 | E04\\ E09 | F04\\ F09 | G04\\ G09 | H04\\ H09 | A05\\ A10 | F05\\ F10 | 4 |
+| 3 | H03\\ H08 | A04\\ A09 | B04\\ B09 | C04\\ C09 | D04\\ D09 | E05\\ E10 | 3 |
+| 2 | C03\\ C08 | D03\\ D08 | E03\\ E08 | F03\\ F08 | G03\\ G08 | D05\\ D10 | 2 |
+| 1 | F02\\ F07 | G02\\ G07 | H02\\ H07 | A03\\ A08 | B03\\ B08 | C05\\ C10 | 1 |
+| 0 | A02\\ A07 | B02\\ B07 | C02\\ C07 | D02\\ D07 | E02\\ E07 | B05\\ B10 | 0 |
+| HY / NX | 0     | 1         | 2         | 3         | 4         |         5 | |
 ==== Freezer Mix ====
-In a 2mL tube:
+In 4x 2mL-tubes:
   * **Tube 2020.09.17-A:** 48 strands A1-H6 plate 1/3 2mL@2mM
   * **Tube 2020.09.17-B:** 48 strands A7-H12 plate 1/3 2mL@2mM
@@ Line 22: / Line 44: @@
 ^  H<sub>2</sub>O ^^^^  ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
 ^ 1 Non-docking strand  |  200000 |  28 |  2000 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+In 2x30 2mL tubes:
+  * **Tube 2020.09.17-Δ//x//://y//** (0,0)≤(//x//,//y//)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM
+  * **Tube 2020.09.17-Δ//x//://y//+** (0,0)≤(//x//,//y//)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM
+^  //1 docking strand Freezer Mix (2mL@5mM) //  ^^^^^^
+^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
+^  one 5mL tube of 1 docking strand ^^^^  2000 ^^
+^  H<sub>2</sub>O ^^^^  ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ 1 docking strand  |  200000 |  1 |  5000 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
@@ Line 32: / Line 66: @@
 ==== Fridge Mix ====
+In 1x 2mL-tube:
+  * **Tube 2020.09.17-E:** all 3x48+28 = 172 strands A1-H12@(plate 1/3)+A1-D10@(plate 2/3) 2mL@100nM
+^  //All 172 Non-docking strands Fridge Mix (2mL@100nM) //  ^^^^^^
+^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
+^  one 2mL tube of all 172 non-docking strands ^^^^  2000 ^^
+^  H<sub>2</sub>O ^^^^  ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ 1 Non-docking strand freezer mix  |  2000 |  4 | 100 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+In 2x30 2mL tubes:
+  * **Tube 2020.09.17-δ//x//://y//** (0,0)≤(//x//,//y//)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@500uM
+  * **Tube 2020.09.17-δ//x//://y//+** (0,0)≤(//x//,//y//)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@500uM
+^  //1 docking strand Fridge Mix (2mL@500uM) //  ^^^^^^
+^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
+^  one 2mL-tube of 1 docking strand ^^^^  2000 ^^
+^  H<sub>2</sub>O ^^^^  ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
+^ 1 docking strand freezer mix  |  5000 |  1 |  500 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+==== Maze Mix ====
+In 500uL-tube:
+^ Y\X  ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^
+^ 0 | o | o | o | o | o | o |
+^ 1 | o | # | o | # | o | # |
+^ 2 | o | o | o | o | o | o |
+^ 3 | # | o | # | o | # | o |
+^ 4 | o | o | o | o | o | o |
+^ Y\X  ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^
+^ 0 | # | o | # | o | # | o |
+^ 1 | o | o | o | o | o | o |
+^ 2 | o | # | o | # | o | # |
+^ 3 | o | o | o | o | o | o |
+^ 4 | # | o | # | o | # | o |
+^ Y\X  ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^
+^ 0 | # | o | # | o | # | o |
+^ 1 | o | # | o | # | o | # |
+^ 2 | # | o | # | o | # | o |
+^ 3 | o | # | o | # | o | # |
+^ 4 | # | o | # | o | # | o |
+^ Y\X  ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^
+^ 0 | o | # | o | # | o | # |
+^ 1 | # | o | # | o | # | o |
+^ 2 | o | # | o | # | o | # |
+^ 3 | # | o | # | o | # | o |
+^ 4 | o | # | o | # | o | # |
 ==== Origami mix ====
-In a 0.5mL tube:
+In a 500uL tube:
-^  //Origami Mix (500uL) //  ^^^^^^
+^  //Origami Mix (50uL) //  ^^^^^^
 ^  Product  ^ Concentration (nM)  ^  Number  ^  Target concentration (nM)  ^  Volume (uL)  ^ <color gray>Vol*nb</color>^
-^  Origami Mix ^^^^  500 ^^
+^  Origami Mix ^^^^  50 ^^
 ^  H<sub>2</sub>O ^^^^  ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^
 ^ TAE Mg 10x  |  10 |  1 |  1 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
 ^ Fridge M13  |  10 |  1 |  1 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
-^ Fridge Mix of 170 non-HPable Staples  |  1000 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+^ Fridge Mix of all 172 non-docking staples  |  100 |  1 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
-^ Fridge of HPable Staples  |  1000 |  42 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |
+^ Fridge mix of each 30 docking staples  |  500 |  30 |  10 |  **~~=cell(3,row())*r2c1/cell(1,row())~~** |  <color gray>~~=cell(4,row())*cell(2,row())~~</color> |