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2020.09.14_water_wings_maze [2020/09/14 15:01] Nicolas Schabanel [Freezer Mix] |
2020.09.14_water_wings_maze [2021/03/05 15:29] (current) |
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==== Plates ==== | ==== Plates ==== | ||
+ | |||
+ | |||
+ | | //A01//\\ - | //A02//\\ (0,0) | //A03//\\ (3,1) | //A04//\\ (1,3) | //A05//\\ (4,4) | //A06//\\ - | //A07//\\ (0,0) | //A08//\\ (3,1) | //A09//\\ (1,3) | //A10//\\ (4,4) | //A11//\\ - | //A12//\\ - | | ||
+ | | //B01//\\ - | //B02//\\ (1,0) | //B03//\\ (4,1) | //B04//\\ (2,3) | //B05//\\ (5,0) | //B06//\\ - | //B07//\\ (1,0) | //B08//\\ (4,1) | //B09//\\ (2,3) | //B10//\\ (5,0) | //B11//\\ - | //B12//\\ - | | ||
+ | | //C01//\\ - | //C02//\\ (2,0) | //C03//\\ (0,2) | //C04//\\ (3,3) | //C05//\\ (5,1) | //C06//\\ - | //C07//\\ (2,0) | //C08//\\ (0,2) | //C09//\\ (3,3) | //C10//\\ (5,1) | //C11//\\ - | //C12//\\ -| | ||
+ | | //D01//\\ - | //D02//\\ (3,0) | //D03//\\ (1,2) | //D04//\\ (4,3) | //D05//\\ (5,2) | //D06//\\ - | //D07//\\ (3,0) | //D08//\\ (1,2) | //D09//\\ (4,3) | //D10//\\ (5,2) | //D11//\\ - | //D12//\\ -| | ||
+ | | //E01//\\ - | //E02//\\ (4,0) | //E03//\\ (2,2) | //E04//\\ (0,4) | //E05//\\ (5,3) | //E06//\\ - | //E07//\\ (4,0) | //E08//\\ (2,2) | //E09//\\ (0,4) | //E10//\\ (5,3) | //E11//\\ - | //E12//\\ -| | ||
+ | | //F01//\\ - | //F02//\\ (0,1) | //F03//\\ (3,2) | //F04//\\ (1,4) | //F05//\\ (5,4) | //F06//\\ - | //F07//\\ (0,1) | //F08//\\ (3,2) | //F09//\\ (1,4) | //F10//\\ (5,4) | //F11//\\ - | //F12//\\ -| | ||
+ | | //G01//\\ - | //G02//\\ (1,1) | //G03//\\ (4,2) | //G04//\\ (2,4) | //G05//\\ - | //G06//\\ - | //G07//\\ (1,1) | //G08//\\ (4,2) | //G09//\\ (2,4) | //G10//\\ - | //G11//\\ - | //G12//\\ -| | ||
+ | | //H01//\\ - | //H02//\\ (2,1) | //H03//\\ (0,3) | //H04//\\ (3,4) | //H05//\\ - | //H06//\\ - | //H07//\\ (2,1) | //H08//\\ (0,3) | //H09//\\ (3,4) | //H10//\\ - | //H11//\\ - | //H12//\\ -| | ||
+ | |||
+ | |||
+ | |||
+ | The maze coordinates: | ||
+ | | HY \ NX | 0 | 1 | 2 | 3 | 4 | 5 | | | ||
+ | | 4 | E04\\ E09 | F04\\ F09 | G04\\ G09 | H04\\ H09 | A05\\ A10 | F05\\ F10 | 4 | | ||
+ | | 3 | H03\\ H08 | A04\\ A09 | B04\\ B09 | C04\\ C09 | D04\\ D09 | E05\\ E10 | 3 | | ||
+ | | 2 | C03\\ C08 | D03\\ D08 | E03\\ E08 | F03\\ F08 | G03\\ G08 | D05\\ D10 | 2 | | ||
+ | | 1 | F02\\ F07 | G02\\ G07 | H02\\ H07 | A03\\ A08 | B03\\ B08 | C05\\ C10 | 1 | | ||
+ | | 0 | A02\\ A07 | B02\\ B07 | C02\\ C07 | D02\\ D07 | E02\\ E07 | B05\\ B10 | 0 | | ||
+ | | HY / NX | 0 | 1 | 2 | 3 | 4 | 5 | | | ||
+ | |||
==== Freezer Mix ==== | ==== Freezer Mix ==== | ||
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In 2x30 2mL tubes: | In 2x30 2mL tubes: | ||
- | * **Tube 2020.09.17-xy(//x//,//y//)** (0,0)≤(//x//,//y//)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM | + | * **Tube 2020.09.17-Δ//x//://y//** (0,0)≤(//x//,//y//)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@5mM |
- | * **Tube 2020.09.17-XY(//x//,//y//)** (0,0)≤(//x//,//y//)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM | + | * **Tube 2020.09.17-Δ//x//://y//+** (0,0)≤(//x//,//y//)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@5mM |
^ //1 docking strand Freezer Mix (2mL@5mM) // ^^^^^^ | ^ //1 docking strand Freezer Mix (2mL@5mM) // ^^^^^^ | ||
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==== Fridge Mix ==== | ==== Fridge Mix ==== | ||
+ | |||
+ | In 1x 2mL-tube: | ||
+ | * **Tube 2020.09.17-E:** all 3x48+28 = 172 strands A1-H12@(plate 1/3)+A1-D10@(plate 2/3) 2mL@100nM | ||
+ | |||
+ | ^ //All 172 Non-docking strands Fridge Mix (2mL@100nM) // ^^^^^^ | ||
+ | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
+ | ^ one 2mL tube of all 172 non-docking strands ^^^^ 2000 ^^ | ||
+ | ^ H<sub>2</sub>O ^^^^ ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
+ | ^ 1 Non-docking strand freezer mix | 2000 | 4 | 100 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
+ | |||
+ | In 2x30 2mL tubes: | ||
+ | * **Tube 2020.09.17-δ//x//://y//** (0,0)≤(//x//,//y//)≤(5,4): 1 unblobbed docking strand/tube from wells A2 to F5 plate 3/3 2mL@500uM | ||
+ | * **Tube 2020.09.17-δ//x//://y//+** (0,0)≤(//x//,//y//)≤(5,4): 1 blobbed docking strand/tube from wells A7 to F10 plate 3/3 2mL@500uM | ||
+ | |||
+ | ^ //1 docking strand Fridge Mix (2mL@500uM) // ^^^^^^ | ||
+ | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
+ | ^ one 2mL-tube of 1 docking strand ^^^^ 2000 ^^ | ||
+ | ^ H<sub>2</sub>O ^^^^ ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
+ | ^ 1 docking strand freezer mix | 5000 | 1 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
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+ | ==== Maze Mix ==== | ||
+ | |||
+ | In 500uL-tube: | ||
+ | |||
+ | ^ Y\X ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ | ||
+ | ^ 0 | o | o | o | o | o | o | | ||
+ | ^ 1 | o | # | o | # | o | # | | ||
+ | ^ 2 | o | o | o | o | o | o | | ||
+ | ^ 3 | # | o | # | o | # | o | | ||
+ | ^ 4 | o | o | o | o | o | o | | ||
+ | |||
+ | |||
+ | ^ Y\X ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ | ||
+ | ^ 0 | # | o | # | o | # | o | | ||
+ | ^ 1 | o | o | o | o | o | o | | ||
+ | ^ 2 | o | # | o | # | o | # | | ||
+ | ^ 3 | o | o | o | o | o | o | | ||
+ | ^ 4 | # | o | # | o | # | o | | ||
+ | |||
+ | ^ Y\X ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ | ||
+ | ^ 0 | # | o | # | o | # | o | | ||
+ | ^ 1 | o | # | o | # | o | # | | ||
+ | ^ 2 | # | o | # | o | # | o | | ||
+ | ^ 3 | o | # | o | # | o | # | | ||
+ | ^ 4 | # | o | # | o | # | o | | ||
+ | |||
+ | |||
+ | ^ Y\X ^ 0 ^ 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ | ||
+ | ^ 0 | o | # | o | # | o | # | | ||
+ | ^ 1 | # | o | # | o | # | o | | ||
+ | ^ 2 | o | # | o | # | o | # | | ||
+ | ^ 3 | # | o | # | o | # | o | | ||
+ | ^ 4 | o | # | o | # | o | # | | ||
==== Origami mix ==== | ==== Origami mix ==== | ||
- | In a 0.5mL tube: | + | In a 500uL tube: |
+ | |||
+ | |||
- | ^ //Origami Mix (500uL) // ^^^^^^ | + | ^ //Origami Mix (50uL) // ^^^^^^ |
^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
- | ^ Origami Mix ^^^^ 500 ^^ | + | ^ Origami Mix ^^^^ 50 ^^ |
^ H<sub>2</sub>O ^^^^ ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ^ H<sub>2</sub>O ^^^^ ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
^ TAE Mg 10x | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ^ TAE Mg 10x | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
^ Fridge M13 | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ^ Fridge M13 | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
- | ^ Fridge Mix of 170 non-HPable Staples | 1000 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | + | ^ Fridge Mix of all 172 non-docking staples | 100 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | |
- | ^ Fridge of HPable Staples | 1000 | 42 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | + | ^ Fridge mix of each 30 docking staples | 500 | 30 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | |