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2021.01.19_m2if_cr11_rocket [2021/01/19 17:55] Nicolas Schabanel [Freezer mix] |
2021.01.19_m2if_cr11_rocket [2021/03/05 15:29] (current) |
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| - | ===== Origami: Rocket 2x layer (M2IF CR11 2021) ===== | + | ===== Origami: Space Invader ===== |
| - | + | ||
| - | ==== IDT plates ==== | + | |
| - | + | ||
| - | 208 stapple strands @200μM each | + | |
| ==== Freezer mix ==== | ==== Freezer mix ==== | ||
| Line 9: | Line 5: | ||
| In 0.5mL tubes: | In 0.5mL tubes: | ||
| - | * I'll make **6x32 = ~~=6*32~~** of those: | + | * I'll make **9x16 = ~~=9*16~~** of those: |
| - | * 2021.01.20A: wells P1:A1-H4 | + | |
| - | * 2021.01.20B: wells P1:A5-H8 | + | |
| - | * 2021.01.20C: wells P1:A9-H12 | + | |
| - | * 2021.01.20D: wells P2:A1-H4 | + | |
| - | * 2021.01.20E: wells P2:A5-H8 | + | |
| - | * 2021.01.20F: wells P2:A9-H12 | + | |
| - | ^ //Freezer Mix of 32 Staples (400uL at 5000nM each) // ^^^^^^ | + | ^ //Freezer Mix of 16 Staples (300uL at 2500nM each) // ^^^^^^ |
| ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Freezer Mix of 35 Staples (5000nM) ^^^^ 400 ^^ | + | ^ Freezer Mix of 16 Staples (2500nM) ^^^^ 300 ^^ |
| ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ staple | 200000 | 32 | 5000 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | + | ^ staple | 50000 | 16 | 2500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | |
| * I'll make 1 of this: | * I'll make 1 of this: | ||
| - | * 2021.01.20G: wells P3:A1-H2 | ||
| - | ^ //Freezer Mix of ~~=208-6*32~~ Staples (400uL at 5000nM each) // ^^^^^^ | + | ^ //Freezer Mix of 12 Staples (300uL at 2500nM each) // ^^^^^^ |
| ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Freezer Mix of ~~=208-6*32~~ Staples (5000nM) ^^^^ 400 ^^ | + | ^ Freezer Mix of 12 Staples (5000nM) ^^^^ 300 ^^ |
| ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ staple | 200000 | ~~=208-6*32~~ | 5000 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | + | ^ staple | 50000 | 12 | 2500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | |
| ==== Fridge mix ==== | ==== Fridge mix ==== | ||
| - | In 0.5mL tube: | + | In 0.5mL tubes: |
| + | |||
| + | * I'll make 1 of these: | ||
| - | ^ //Fridge Mix of ~~=6*32+16~~ Staples (500uL at 500nM) // ^^^^^^ | + | ^ //Fridge Mix of 80 Staples (500uL at 500nM) // ^^^^^^ |
| ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Fridge Mix of 6x32+16=~~=6*32+16~~ Staples (500nM) ^^^^ 500 ^^ | + | ^ Fridge Mix of 80 Staples (1000nM) ^^^^ 500 ^^ |
| ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| - | ^ Freezer Mix of 32 Staples (5000nM) | 5000 | 6 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | + | ^ Freezer Mix of 16 Staples (2500nM) | 2500 | 5 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | |
| - | ^ Freezer Mix of 16 Staples (5000nM) | 5000 | 1 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | + | |
| + | * I'll make 1 of these: | ||
| + | |||
| + | ^ //Fridge Mix of 76 Staples (500uL at 500nM) // ^^^^^^ | ||
| + | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| + | ^ Fridge Mix of ~~=4*16+12~~ Staples (1000nM) ^^^^ 500 ^^ | ||
| + | ^ H<sub>2</sub>O ^^^^ **~~=r2c1-sum(r4c5:r99c5)~~** ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| + | ^ Freezer Mix of 16 Staples (2500nM) | 2500 | 4 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| + | ^ Freezer Mix of 12 Staples (2500nM) | 2500 | 1 | 500 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| ==== M13 fridge tube ==== | ==== M13 fridge tube ==== | ||
| Line 59: | Line 57: | ||
| ^ //Origami Mix (500uL) // ^^^^^^ | ^ //Origami Mix (500uL) // ^^^^^^ | ||
| ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ^ Product ^ Concentration (nM) ^ Number ^ Target concentration (nM) ^ Volume (uL) ^ <color gray>Vol*nb</color>^ | ||
| - | ^ Origami Mix ^^^^ 50 ^^ | + | ^ Origami Mix ^^^^ 500 ^^ |
| ^ H<sub>2</sub>O ^^^^ ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ^ H<sub>2</sub>O ^^^^ ~~=r2c1-sum(r4c5:r99c5)~~ ^ ~~=check(compare(r3c1,0,#>=),#Ok,#FIXME)~~ ^ | ||
| ^ TAE Mg 10x | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ^ TAE Mg 10x | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| ^ Fridge M13 | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ^ Fridge M13 | 10 | 1 | 1 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||
| - | ^ Fridge Mix of 208 Staples | 500 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | + | ^ Fridge Mix of 80 Staples | 500 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | |
| + | ^ Fridge Mix of 76 Staples | 500 | 1 | 10 | **~~=cell(3,row())*r2c1/cell(1,row())~~** | <color gray>~~=cell(4,row())*cell(2,row())~~</color> | | ||